16 Jan #InBrief: Calprotectin
The rapidly growing global challenge of antibiotic resistance, demands a more specific and reduced use of antibiotics. It is therefore important to have markers that distinguish well between bacterial and viral infections.
In clinical practice, the diagnosis of a bacterial infection is made from observation of clinical symptoms, supported by leukocyte counts and/or C-reactive Protein (CRP) measurement. Still, it has been estimated that about 40 % of cases misclassify between bacterial infections and other causes .
Neutrophil activation markers such as calprotectin, alone or in combination with other inflammation markers, can potentially be superior to e.g. CRP to distinguish between bacterial and viral infections.
Calprotectin – a neutrophil activation marker
Calprotectin is a soluble proinflammatory mediator, which is secreted from neutrophil immune cells during pro-inflammatory response [2,3]. The proinflammatory mediator is stored in the granules of neutrophils, where it accounts for approximately 30–40 % of the cytosol protein content. Therefore, it works well as a marker for neutrophil activation. It elevates in inflammatory conditions where neutrophil activation is involved, and is therefore an important marker for inflammatory diseases. .
Calprotectin to monitor disease development
The concentration of calprotectin reflects the degree of inflammation, because calprotectin levels rise proportionally with the degree of inflammation . Thus, monitoring calprotectin allows surveillance of disease development and treatment response .
GCAL – Gentian Calprotectin Immunoassay
Gentian develops diagnostic tests that are accurate and easy to implement. The Gentian Calprotectin Immunoassay allows for rapid and flexible random access use. It is also an ideal solution for high throughput applications in routine laboratories. The assay can be applied on all automated platforms in clinical chemistry. Equally important, it allows short turnaround time from sample to reportable results.
CE Marked 15th July 2017
Developed and manufactured by Gentian
First and only turbidimetric immunoassay for analysing calprotectin in plasma and serum (patented by Gentian)
- Xu S, Venge P. Lipocalins as biochemical markers of disease. Biochim Biophys Acta. 2000;1482:298–307
- Hessian, PA et al (1993) J Leukoc Biol 53: 197-204
- Yui, S et al (1995) J leukoc Biol 58: 650-658
- Celio MR et al. Oxford Univ Press 1996, pp. 147–148
- Fagerhol M K, Nielsen H G, Vetlesen A, Sandvik K, Lyberg T. Increase in plasma calprotectin during long‐distance running. Scand J Clin Lab Invest 200565211–220
This article is based on A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analyzers, Nilsen et al. Journal of Inflammation (2015) 12:45 DOI 10.1186/s12950-015-0090-3.