FAQ Plasma Calprotectin Immunoassay | Inflammation | Gentian
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FAQ: Gentian Calprotectin (GCAL)

Calprotectin - bacterial infections

FAQ: Gentian Calprotectin (GCAL)

FAQ: Gentian Calprotectin (GCAL)

Calprotectin is a soluble proinflammatory mediator secreted from neutrophil immune cells during the proinflammatory response [2,3]. Calprotectin is stored in the granules of neutrophils. Research shows that the concentration of calprotectin reflects the degree of inflammation, and treatment efficiency can potentially be monitored due to the short half-life of calprotectin in serum and plasma (5 hours) [5]

What is the Gentian Calprotectin Immunoassay?

The Gentian Calprotectin Immunoassay is a turbidimetric assay for measurement of calprotectin in plasma samples. It allows for rapid and flexible random access use, as well as being the ideal solution for high throughput applications in the routine laboratory. The assay can be applied on all automated platforms in clinical chemistry and allows short turnaround time from sample to reportable result.

Calprotectin levels are higher in plasma and serum of patients with sepsis and in patients admitted for postoperative care after major surgery, than in patients admitted due to intoxication, suggesting that calprotectin levels are markedly increased in systemic inflammatory response. Plasma calprotectin accurately discriminates presence versus absence of systemic inflammation.


Measurement of calprotectin in plasma 
in the diagnosis of inflammation

How does it compare to current methods?

Initial studies show that GCAL together with C-reactive protein (CRP) have a high predictive value for identifying sepsis vs post-operative inflammation than either marker separately [4]. Since CRP and procalcitonin are established biomarkers of sepsis, we recommend that GCAL is added to current diagnostic routines.

Calprotectin in diagnostic, plasma calprotectin

Are there other uses?

Potentially, as calprotectin is a marker for the diagnosis of inflammation it has also been shown to be useful in the diagnosis of rheumatoid arthritis [5], appendicitis [6] and systemic sclerosis [7]. Clinical studies using this assay are needed to further determine clinical use.

Why measure calprotectin in sepsis?

Sepsis is the leading cause of death in Intensive Care Unit (ICU) patients [1]. Changes in serum calprotectin levels can supply rapid information in patients with sepsis upon ICU admission and indicate treatment effectiveness [8].


  1. Huang, L et al (2016) J Interf Cytok Res 36, 607-616
  2. Hessian, PA et al (1993) J Leukoc Biol 53: 197-204
  3. Yui, S et al (1995) J leukoc Biol 58: 650-658
  4. Simm, M et al (2016) Biomark Med 10, 811-818
  5. Nordal, HH et al (2017) Arthritis Res Ther 19:3
  6. Cikot, M et al (2016) World J Emerg Surg 11:17
  7. van Bon, L et al (2014) Ann Rheum Dis 73:1585-1589
  8. Natesan, M et al (2017) J Clin Microbiol 55:1205-12